Journal article
Downregulation of type I interferon signalling pathway by urate in primary human PBMCs
M Badii, V Nica, AR Straton, B Kischkel, O Gaal, G Cabău, V Klück, I Hotea, LAB Joosten, IV Pop, RA Popp, S Rednic, C Pamfil, TO Crişan, M Farcaş†, DH Marginean, L Peca, AM Mirea, D Colcear, MS Pop Show all
Immunology | WILEY | Published : 2025
DOI: 10.1111/imm.13858
Abstract
Type I interferons (IFN1s) mediate innate responses to microbial stimuli and regulate interleukin (IL)-1 and IL-1 receptor antagonist (Ra) production in human cells. This study explores interferon-stimulated gene (ISG) alterations in the transcriptome of patients with gout and stimulated human primary cells in vitro in relation to serum urate concentrations. Peripheral blood mononuclear cells (PBMCs) and monocytes of patients with gout were primed in vitro with soluble urate, followed by lipopolysaccharide (LPS) stimulation. Separately, PBMCs were stimulated with various toll-like receptor (TLR) ligands. RNA sequencing and IL-1Ra cytokine measurement were performed. STAT1 phosphorylation was..
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Grants
Awarded by Ministerul Cercetării, Inovării şi Digitalizării
Funding Acknowledgements
Competitiveness Operational Programme grant of the Romanian Ministry of European Funds, Grant/Award Numbers: P_37_762, MySMIS 103587; Romanian Ministry of European Investments and Projects, Grant/Award Numbers: PNRR-III- C9-2022-I8, CF 85/15.11.2022; Ministry of Research, Innovation and Digitization, CNCS/CCCDI - UEFISCDI, Grant/Award Numbers: PD-2019-0802, TE-2021-1512; "Iuliu Hatieganu" University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania, Grant/Award Number: 1529/6 PCD; PhD grant of the Radboudumc; ERC Advanced Grant, Grant/Award Number: 833247; Spinoza Grant of the Netherlands Organization for Scientific Research; Australia NHMRC Investigator Grant, Grant/Award Number: 1173314